3D/4D Bioprinting

High resolution 3D printing of volumetric structures –

We developed a uniquely formulated media used to support printing of extracellular matrix-based biomaterials. The hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures. This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.

3D confocal image of a double layered construct, printed in the support medium. 1mm

A macroscopic view of printed structures after extraction from the support medium. 5mm

Accurate, high resolution thick structures printed from the personalized hydrogel.

3D confocal image of a double layered construct, printed in the support medium. 1mm

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Printed cardiac patches are physically robust and can be handled easily.

Cross‐section of a printed lumen. Green: ECs; red: fibroblasts.

Cross‐sections of the heart immunostained against sarcomeric actinin (green). 1mm

Printed cardiac patches are physically robust and can be handled easily.

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3D printing of patient specific tissues and organs –

Our lab develops technologies to print specific patient-derived hydrogel and cells. One of our long-term goals is to recapitulate the heart function by replacing the infarcted organ with a 3D-printed version of a functional heart.

4D printing of dynamic living tissues –

Our lab develops advanced 4D bioprinting technologies for engineering dynamic living tissues with programmable structural and functional transformations over time. By integrating smart biomaterials, extracellular matrix-based bioinks, stem cell-derived tissues, and multi-material biofabrication strategies, we aim to create complex tissue architectures that better mimic native tissue organization.